By Henry Tedeschi
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Additional info for Cell physiology: molecular dynamics
Cell Organization: Techniques and a Preliminary View A number of variations of the original two-hybrid approach have been developed for the study of nucleic acid-protein interactions (see Vidal and Legrain, 1999) (for more details click here). , 2000) have been used in the study of the interactions between DNA and proteins. In immunoprecipitation formaldehyde is used to crosslink the proteins to the DNA. Then the crosslinked material is precipitated immunologically. The DNA microarray technique follows the same general concept used for studying DNA-RNA binding.
And Porter, K. R. (1967) Cytoplasmic fibril in living cultured cells. R. , Mian, N. and Bateman, A.
These procedures are simpler and have proven very practical. When injected into mice, PCR-amplified DNA open-reading frames (ORFs) noncovalently linked to a eukaryotic promoter and terminator, produce antibodies specific for the encoded protein (Sykes and Johnston, 1999). Monoclonal antiodies can also be produced by display technology. In the immune system the rearrangement of V gene segments produces the repertoire of antibodies, each capable of interacting with one antigen. These gene segments can be amplified using PCR.