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By Valerie DeBenedette

Examines the background, actual results, and social features of caffeine, the main everyday drug on this planet. summary: Examines the heritage, actual results, and social elements of caffeine, the main commonplace drug on the planet

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1998 CRC Press LLC FIGURE 2 The tea flavanols. 2. Flavonols and Glycosides These substances are present in small amounts in tea leaf but there is little quantitative data on their occurrence. Figure 3 shows the structure of several of these that have been identified. They occur both as free flavonols and glycosides. 24 They may enter into the oxidative reactions that take place during tea manufacture. 50 3. Gallic Acid Gallic acid is present in tea leaf and is a known reactant during the complex enzymatic and organochemical reactions that occur when tea components are oxidized.

Determination of catechins and methylxanthines in foods by semi-micro HPLC, J. Food Hygienic Soc. , 33(4),347,1992 33. Coffee. Determination of the caffeine content, HPLC method, Association Francaise de Normalisation French Standard, France, NF ISO 10095, 1992. 35. Methods of test for coffee and coffee products. Part 12. Coffee: determination of caffeine content (routine method by HPLC), British Standards Institution, United Kingdom, British-Standard, BS 5752:Part 12, 1992. 36. , Determination of aspartame, acesulfam-K, saccharin, caffeine, sorbic acid and benzoic acid in foods by HPLC.

After centrifugation, the aqueous layer is discarded and the chloroform layer is dried under nitrogen. Five milliliters toluene are added in addition to ammonium hydroxide. The mixture is again centrifuged, the toluene layer is discarded, and the aqueous phase evaporated to dryness. 4% tetramethylammonium hydroxide in methanol. The derivatizing agent, ©1998 CRC Press LLC iodobutane, is added and then the solution is mixed. The method is specific for theophylline and presents excellent data. As in the case in the analysis of food samples, the introduction of relatively inexpensive MS detectors for GC has had a substantial impact on the determination of methylxanthines by GC.

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